Cocoa: Detection and determination of theobromine and caffeine in urine after administration of chocolate-coated peanuts to horses
Home Page For The "Cocoa Source For Mood Enhancers"
Detection and determination of theobromine and caffeine in urine after administration of chocolate-coated peanuts to horses
by
Dyke TM, Sams RA
Analytical Toxicology Laboratory,
College of Veterinary Medicine,
Ohio State University, Columbus 43210-1089, USA.
J Anal Toxicol 1998 Mar-Apr; 22(2):112-6
ABSTRACTT
he objective of this study was to determine the urinary excretion of methylxanthines in horses following ingestion of chocolate over eight days.The study was performed in response to gas chromatography-mass spectrometry (GC-MS) confirmation of the presence of caffeine in a positive urine test in a racehorse. The trainer of the horse alleged that he often administered chocolate-coated peanuts as treats to his horses, and he believed that the ingestion of chocolate was responsible for the positive urine test.
The urinary excretion of theobromine and caffeine after the ingestion of chocolate-coated peanuts was investigated in three horses.
Enzyme-linked immunoassay (ELISA), high-performance liquid chromatography (HPLC), and GC-MS assays were performed on all urine specimens.
Theobromine (HPLC) was detected for 72 h and caffeine (GC-MS) for 48 h after chronic ingestion of chocolate-coated peanuts.
Methylxanthines were detected by ELISA for 120 h after administration of chocolate.
Karl Note: I reject strongly the notion that a "mood booster" can ever be some chemical substance, whether a psychiatric drug or a so-called "natural" substance. The belief that "mood" can be changed with chemicals is the original contribution of the "Man Came From Mud" theory, and supports the entire "allopathic" system of medicine that holds that drugs are the key to health! This is a false path down which to travel. Vibrant Life feels so strongly about this that we refuse to sell any of our products to any person taking any "mood enhancer" substance, whether psychiatric drug, illegal street drug or otherwise.
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